Genome:

Chr:

Start:

End:

gRNA length:

Formulation:

Constraint:

PAM sequence:

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Mismatches allowed:

Off target window:

Off target ratio:

Minimal gap:

Excluded substring:

Modify binding scores (mismatch and pam scores)

Mismatch score:

rA:dA,1=1.0
rA:dA,10=0.882352941
rA:dA,11=0.307692308
rA:dA,12=0.333333333
rA:dA,13=0.3
rA:dA,14=0.533333333
rA:dA,15=0.2
rA:dA,16=0.0
rA:dA,17=0.133333333
rA:dA,18=0.5
rA:dA,19=0.538461538
rA:dA,2=0.727272727
rA:dA,20=0.6
rA:dA,3=0.705882353
rA:dA,4=0.636363636
rA:dA,5=0.363636364
rA:dA,6=0.7142857140000001
rA:dA,7=0.4375
rA:dA,8=0.428571429
rA:dA,9=0.6
rA:dC,1=1.0
rA:dC,10=0.5555555560000001
rA:dC,11=0.65
rA:dC,12=0.7222222220000001
rA:dC,13=0.6521739129999999
rA:dC,14=0.46666666700000003
rA:dC,15=0.65
rA:dC,16=0.192307692
rA:dC,17=0.176470588
rA:dC,18=0.4
rA:dC,19=0.375
rA:dC,2=0.8
rA:dC,20=0.764705882
rA:dC,3=0.611111111
rA:dC,4=0.625
rA:dC,5=0.72
rA:dC,6=0.7142857140000001
rA:dC,7=0.705882353
rA:dC,8=0.7333333329999999
rA:dC,9=0.666666667
rA:dG,1=0.857142857
rA:dG,10=0.333333333
rA:dG,11=0.4
rA:dG,12=0.263157895
rA:dG,13=0.21052631600000002
rA:dG,14=0.214285714
rA:dG,15=0.272727273
rA:dG,16=0.0
rA:dG,17=0.176470588
rA:dG,18=0.19047619
rA:dG,19=0.20689655199999998
rA:dG,2=0.7857142859999999
rA:dG,20=0.22727272699999998
rA:dG,3=0.428571429
rA:dG,4=0.352941176
rA:dG,5=0.5
rA:dG,6=0.454545455
rA:dG,7=0.4375
rA:dG,8=0.428571429
rA:dG,9=0.571428571
rC:dA,1=1.0
rC:dA,10=0.9411764709999999
rC:dA,11=0.307692308
rC:dA,12=0.538461538
rC:dA,13=0.7
rC:dA,14=0.7333333329999999
rC:dA,15=0.066666667
rC:dA,16=0.307692308
rC:dA,17=0.46666666700000003
rC:dA,18=0.642857143
rC:dA,19=0.46153846200000004
rC:dA,2=0.9090909090000001
rC:dA,20=0.3
rC:dA,3=0.6875
rC:dA,4=0.8
rC:dA,5=0.636363636
rC:dA,6=0.9285714290000001
rC:dA,7=0.8125
rC:dA,8=0.875
rC:dA,9=0.875
rC:dC,1=0.913043478
rC:dC,10=0.38888888899999996
rC:dC,11=0.25
rC:dC,12=0.444444444
rC:dC,13=0.13636363599999998
rC:dC,14=0.0
rC:dC,15=0.05
rC:dC,16=0.153846154
rC:dC,17=0.058823529000000006
rC:dC,18=0.133333333
rC:dC,19=0.125
rC:dC,2=0.695652174
rC:dC,20=0.058823529000000006
rC:dC,3=0.5
rC:dC,4=0.5
rC:dC,5=0.6
rC:dC,6=0.5
rC:dC,7=0.470588235
rC:dC,8=0.642857143
rC:dC,9=0.6190476189999999
rC:dT,1=1.0
rC:dT,10=0.8666666670000001
rC:dT,11=0.75
rC:dT,12=0.7142857140000001
rC:dT,13=0.384615385
rC:dT,14=0.35
rC:dT,15=0.222222222
rC:dT,16=1.0
rC:dT,17=0.46666666700000003
rC:dT,18=0.538461538
rC:dT,19=0.428571429
rC:dT,2=0.727272727
rC:dT,20=0.5
rC:dT,3=0.8666666670000001
rC:dT,4=0.842105263
rC:dT,5=0.571428571
rC:dT,6=0.9285714290000001
rC:dT,7=0.75
rC:dT,8=0.65
rC:dT,9=0.857142857
rG:dA,1=1.0
rG:dA,10=0.8125
rG:dA,11=0.384615385
rG:dA,12=0.384615385
rG:dA,13=0.3
rG:dA,14=0.26666666699999997
rG:dA,15=0.14285714300000002
rG:dA,16=0.0
rG:dA,17=0.25
rG:dA,18=0.666666667
rG:dA,19=0.666666667
rG:dA,2=0.636363636
rG:dA,20=0.7
rG:dA,3=0.5
rG:dA,4=0.363636364
rG:dA,5=0.3
rG:dA,6=0.666666667
rG:dA,7=0.571428571
rG:dA,8=0.625
rG:dA,9=0.533333333
rG:dG,1=0.7142857140000001
rG:dG,10=0.4
rG:dG,11=0.428571429
rG:dG,12=0.529411765
rG:dG,13=0.42105263200000004
rG:dG,14=0.428571429
rG:dG,15=0.272727273
rG:dG,16=0.0
rG:dG,17=0.235294118
rG:dG,18=0.47619047600000003
rG:dG,19=0.448275862
rG:dG,2=0.692307692
rG:dG,20=0.428571429
rG:dG,3=0.384615385
rG:dG,4=0.529411765
rG:dG,5=0.7857142859999999
rG:dG,6=0.681818182
rG:dG,7=0.6875
rG:dG,8=0.615384615
rG:dG,9=0.538461538
rG:dT,1=0.9
rG:dT,10=0.933333333
rG:dT,11=1.0
rG:dT,12=0.933333333
rG:dT,13=0.923076923
rG:dT,14=0.75
rG:dT,15=0.9411764709999999
rG:dT,16=1.0
rG:dT,17=0.933333333
rG:dT,18=0.692307692
rG:dT,19=0.7142857140000001
rG:dT,2=0.846153846
rG:dT,20=0.9375
rG:dT,3=0.75
rG:dT,4=0.9
rG:dT,5=0.8666666670000001
rG:dT,6=1.0
rG:dT,7=1.0
rG:dT,8=1.0
rG:dT,9=0.642857143
rU:dC,1=0.956521739
rU:dC,10=0.5
rU:dC,11=0.4
rU:dC,12=0.5
rU:dC,13=0.260869565
rU:dC,14=0.0
rU:dC,15=0.05
rU:dC,16=0.346153846
rU:dC,17=0.117647059
rU:dC,18=0.333333333
rU:dC,19=0.25
rU:dC,2=0.84
rU:dC,20=0.176470588
rU:dC,3=0.5
rU:dC,4=0.625
rU:dC,5=0.64
rU:dC,6=0.571428571
rU:dC,7=0.588235294
rU:dC,8=0.7333333329999999
rU:dC,9=0.6190476189999999
rU:dG,1=0.857142857
rU:dG,10=0.533333333
rU:dG,11=0.666666667
rU:dG,12=0.947368421
rU:dG,13=0.7894736840000001
rU:dG,14=0.28571428600000004
rU:dG,15=0.272727273
rU:dG,16=0.666666667
rU:dG,17=0.705882353
rU:dG,18=0.428571429
rU:dG,19=0.275862069
rU:dG,2=0.857142857
rU:dG,20=0.090909091
rU:dG,3=0.428571429
rU:dG,4=0.647058824
rU:dG,5=1.0
rU:dG,6=0.9090909090000001
rU:dG,7=0.6875
rU:dG,8=1.0
rU:dG,9=0.923076923
rU:dT,1=1.0
rU:dT,10=0.857142857
rU:dT,11=0.75
rU:dT,12=0.8
rU:dT,13=0.692307692
rU:dT,14=0.6190476189999999
rU:dT,15=0.578947368
rU:dT,16=0.9090909090000001
rU:dT,17=0.533333333
rU:dT,18=0.666666667
rU:dT,19=0.28571428600000004
rU:dT,2=0.846153846
rU:dT,20=0.5625
rU:dT,3=0.7142857140000001
rU:dT,4=0.47619047600000003
rU:dT,5=0.5
rU:dT,6=0.8666666670000001
rU:dT,7=0.875
rU:dT,8=0.8
rU:dT,9=0.9285714290000001

PAM score: